DOI - Mendel University Press

DOI identifiers

ISBN online: 978-80-7509-947-1 | DOI: 10.11118/978-80-7509-947-1

METODIKA KVANTIFIKACE HOUBOVÉHO PATOGENU Rhizoctonia solani V PŮDĚ METODOU REAL-TIME PCR

Certifikovaná metodika

Ludmila Holková, Barbora Jílková, Jana Víchová, Martin Kmoch

The aim of the certified methodology is to optimize molecular methods of detection and quantification of the polyphagous fungal pathogen Rhizoctonia solani from the soil environment. The methodology is focused on potato host, in which this pathogen causes a significant disease – black scurf of potato. The SybrGreen real-time PCR method used enables high sensitivity of the detection of the mentioned pathogen in the soil and ensures the reliability of the diagnosis. The protocols are optimized for routine use in molecular biology laboratories.

Keywords: Solanum tuberosum, black scurf of potato, qPCR, relativ quantification

1. edition, online: 2023, publisher: Mendelova univerzita v Brně



References

  1. Ackermann, P., Talich, P., Řehák, V., Kocourek, F. (ed.). (2013). Metodická příručka integrované ochrany rostlin proti chorobám, škůdcům a plevelům. Praha: Česká společnost rostlinolékařská. ISBN 978-80-02-02480-4.
  2. Ajayi-Oyetundea, O.O., Bradley, C.A. (2018). Rhizoctonia solani: taxonomy, population biology and management of rhizoctonia seedling disease of soybean. Plant Pathology, 67: 3-17. https://doi.org/10.1111/ppa.12733 Go to original source...
  3. Anguiz, R., Martin, C. (1989). Anastomosis groups, pathogenicity and other characteristics of Rhizoctonia solani isolated from potato in Peru. Plant Disease, 73: 199-201. Go to original source...
  4. Balali, G.R., Neate, S.M., Scott, E.S., Whisson, D.L., Wicks, T.J. (1995). Anastomosis group and pathogenicity of isolates of Rhizoctonia solani from potato crops in south Australia. Plant Pathology, 44: 1050-1057. Go to original source...
  5. Cullen, D.W., Lees, A.K., Toth, I.K., Duncan, J.M. (2001). Conventional PCR and real-time quantitative PCR detection of Helminthosporium solani in soil and on potato tubers. European Journal of Plant Pathology, 107: 387-398. https://doi.org/10.1023/A:1011247826231 Go to original source...
  6. Dubey, S.C., Tripathi, A., Upadhyay, B.K., Kumar, A. (2016). Development of conventional and real time PCR assay for detection and quantification of Rhizoctinia solani infecting pulse crops. Biologia 71: 133-138. https://doi.org/10.1515/biolog-2016-0027 Go to original source...
  7. Lees, A.K., Cullen, D.W., Sullivan, L., Nicolson, M.J. (2002). Development of conventional and quantitative real-time PCR assays for the detection and identification of Rhizoctonia solani AG-3 in potato and soil. Plant Pathology, 51: 293-302. https://doi.org/10.1046/j.1365-3059.2002.00712.x Go to original source...
  8. Lievens, B., Brouwer, M., Vanachter, A.C.R.C., Cammue, B.P.A., Thomma, B.P.H.J. (2006). Real-time PCR for detection and quantification of fungal and oomycete tomato pathogens in plant and soil samples. Plant Science, 175 (1): 155-175. https://doi.org/10.1016/j.plantsci.2006.03.009 Go to original source...
  9. Mičuda, S., Fuksa, L., Brčáková, E., Cermanová, J., Geršl, V. (2006). Molekulárně biologické metodiky ve farmakologii [online]. Ústav farmakologie, Univerzita Karlova v Praze, Lékařská fakulta v Hradci Králové, [cit. 18. dubna 2022].
  10. Okubara, P.A., Schroeder, K.L., Paulitz T.C. (2008). Identification and quantification of Rhizoctonia solani and R. oryzae using Real-Time Polymerase Chain Reaction. Phytopathology, 98: 837-847. https:// doi.org/10.1094/PHYTO-98-7-0837 Go to original source...
  11. Pfaffl, M.W. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 29(9): e45. https://doi.org/10.1093/nar/29.9.e45 Raclavský, V. (2003). Metody molekulární genetiky. [online]. [cit. 23. dubna. 2022]. Dostupné na www: http://biologie.upol.cz/metody/ Go to original source...
  12. Rasmuseen, R. (2001). Quantification on the LightCycler, s. 1-34. In: Meuer, S., Wittwer, C., Nakagawaran, K.I.: Rapid Cycle Real-Time PCR. Springer, Berlin, Heidelberg. https.//doi.org/10.1007/978-3-642-59524-0_3 Go to original source...
  13. Taberlet, P., Gielly, L., Pautou, G., Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 17: 1105-1109, https://doi.org/10.1007/BF00037152 Go to original source...
  14. Wallon, T., Sauvageau, A., Van der Heyden, H. (2020). Detection and quantification of Rhizoctonia solani and Rhizoctonia solani AG1-IB causing the bottom rot of lettuce in tissues and soils by multiplex qPCR. Plants, 10(1): 57. https://doi.org/10.33804/pp.005.03.3925 Go to original source...
  15. Woodhall, J.W., Adams, I.P., Peters, J.C., Harper, G., Boonham, N. (2013). A new quantitative real-time PCR assay for Rhizoctonia solani AG3-PT and the detection of AGs of Rhizoctonia solani associated with potato in soil and tuber samples in |Great Britain. European Journal of Plant Pathology, 136: 273-280. https://doi.org/10.1007/s10658-012-0161-8 Go to original source...
  16. Žižka J. (2022). Situační a výhledová zpráva brambory. Ministerstvo zemědělství. s. 52. ISBN 978-80-7434-655-2
  17. Kmoch, M., Holková, L., Pokorný, R., Cerkal, R. (2013). Identifikace a kvantifikace hub rodu Fusarium v obilkách kukuřice a ječmene pomocí metody založené na qPCR. Certifikovaná metodika. První vydání. Mendelova univerzita v Brně: Ediční středisko Mendelovy univerzity v Brně. 27 s. ISBN: 978-80-7375-909-4
  18. Holková, L., Jílková, B., Víchová, J., Bačová N., Kmoch, M. (2023) Možnosti detekce a kvantifikace Rhizoctonia solani v půdě. Úroda, 12(LXX), vědecká příloha, 93-100. ISSN 0139-6013