DOI: 10.11118/978-80-7509-994-5-0007

EFFECT OF CARBON MATERIAL AND BENTONITE ON REDUCTION THE NEGATIVE IMPACTS OF DEOXYNIVALENOL IN RAT ORGANISM

TEREZA AULICHOVÁ¹, SYLVIE SKALIČKOVÁ¹, TOMÁŠ KOPEC², PAVEL HORKݹ

¹ Department of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel university in Brno, Zemědělská 1, 613 00 Brno, Czech Republic

² Department of Animal Breeding and Breeding, Faculty of AgriSciences, Mendel university in Brno, Zemědělská 1, 613 00 Brno, Czech Republic$2

The aim of this study was to investigate the efficacy of mycotoxin adsorbents based on graphene oxide (GO), purified bentonites, and their combination in reducing the negative impact of deoxynivalenol (DON). The experiment involved 48 rats (190 ± 5 g), divided into 8 groups of 6 each. Groups one to seven received a diet containing a high level of DON. Groups one to six had their feed treated with mycotoxin adsorbent. Group one (T1) received a diet with GO (0.25 g/kg), group two (T2) a diet with GO (0.5 g/kg), group three (T3) a diet with purified bentonite (1 g/kg), group four (T4) a diet with purified bentonite (2 g/kg), group five (T5) a diet with GO (0.25 g/kg) and purified bentonite (1.75 g/kg), and group six (T6) a diet with GO (0.5 g/kg) and purified bentonite (1.5 g/kg). The control group (C) was fed a diet without DON. The Cmyko group received a diet with mycotoxins without adsorbent. Evaluated parameters included weight and liver enzymes (alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). No statistically significant difference in body weight was observed between groups C and T1–T6 or between groups Cmyko and T1–T6. For liver enzymes, no statistically significant differences were observed in ALP. ALT activity was higher in groups T1, T4, T5, T6, and Cmyko compared to group C. AST activity showed statistically significant differences in all test groups (T1–T6) compared to groups Cmyko and C.In conclusion, a higher dose of GO (0.5 g/kg), purified bentonite,and their combination may be beneficial in mitigating the toxicity ofDON.

Klíčová slova: mycotoxins; bentonite; graphene oxide

stránky: 7-21, online: 2024