DOI - Vydavatelství Mendelovy univerzity v Brně

Identifikátory DOI

DOI: 10.11118/978-80-7509-994-5-0059

EFFECT OF BACTERIAL INOCULANT ON MYCOTOXIN CONTAMINATION OF ALFALFA SILAGE

MATÚŠ DŽIMA1, MIROSLAV JURÁČEK1, DANIEL BÍRO1, MILAN ŠIMKO1, BRANISLAV GÁLIK1, MICHAL ROLINEC1, ONDREJ HANUŠOVSKÝ1, MÁRIA KAPUSNIAKOVÁ1, ELA TARIŠKOVÁ1, ADRIANA PÍŠOVÁ1
1 Institute of Nutrition and Genomics, Department of Animal Nutrition, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovak Republic

The study aimed to investigate the hygienic quality of alfalfa silages, with a focus on the observed concentrations of mycotoxins. It also examined the effect of biological additives, based on homo- and heterofermentative strains of lactic acid bacteria, on the mycotoxin contamination of alfalfa silages. The study monitored several mycotoxins, including total ochratoxins (OTA), total aflatoxins (AFL), total fumonisins (FUM), deoxynivalenol (DON), zearalenone (ZEA), and T-2 toxin (T-2). Mycotoxin concentrations were determined by spectrophotometric immunoenzymatic method (Elisa Reader, Noack SR; Veratox assays, Neogen Ltd., USA) at a wavelength of 650 nm, in alfalfa silage samples that were ensiled into silage units in a volume of 3.5 dm3. The samples were ensiled in control variant C, without the addition of additive, and in experimental variant A with the addition of biological additive (Lactobacillus plantarum, Lactobacillus buchneri) 1.3 x 1011 KTJ.g-1 at a dose of 10 ml per ton. Both control and experimental treatments were fed in 3 replications (n=3). Analysis of mycotoxic contamination of alfalfa silages revealed statistically significantly (p ˂ 0.05) lower fumonisin content in the experimental variant (47.08 μg.kg-1) compared to the control (70.77 μg.kg-1), with a difference of 33.48%. Also, a lower aflatoxin content (3.69 μg.kg-1) was found in the experimental variant compared to the control (4.03 μg.kg-1), which was 8.44%, but these differences were not statistically significant. The contents of DON, ZEA, T-2 and OTA were higher in the experimental variant compared to the control. The mean mycotoxin content of the samples studied showed that alfalfa silages were the most contaminated with ZEA (360.00 μg.kg-1), followed by DON (209.30 μg.kg-1), T-2 toxin (80.38 μg.kg-1), FUM (58.92 μg.kg-1), OTA (53.37 μg.kg-1) and were the least contaminated with AFL (3.81 μg.kg-1). The concentrations of the monitored mycotoxins in alfalfa silages did not exceed the limit values applicable in the EU, which is a prerequisite for ensuring efficient and safe production of animal products.

Klíčová slova: alfalfa silage; mycotoxins; hygienic quality; bacterial inoculant

stránky: 59-73, online: 2024



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