DOI - Mendel University Press

DOI identifiers

DOI: 10.11118/978-80-7509-996-9-0072

VYUŽITÍ CHROMATOGRAFICKÝCH KOLON S NÍZKÝM ZRNĚNÍM PŘI ANALÝZE REZIDUÍ β-LAKTAMOVÝCH A TETRACYKLINOVÝCH ANTIBIOTIK / THE USE OF SUB-2 μM CHROMATOGRAPHIC COLUMNS IN THE ANALYSIS OF β-LACTAM AND TETRACYCLINE ANTIBIOTIC RESIDUES

Eva Dadáková1, Kateřina Matějková1, Hana Kadlecová1, Simona Janoušek Honesová2, Eva Samková2
1 Katedra aplikované chemie, Fakulta zemědělská a technologická, Jihočeská univerzita v Českých Budějovicích, Studentská 1668, 37005 České Budějovice
2 Katedra potravinářských biotechnologií a kvality zemědělských produktů, Fakulta zemědělská a technologická, Jihočeská univerzita v Českých Budějovicích, Studentská 1668, 37005 České Budějovice

The study tested the possibility of replacing classic chromatographic columns with a column with a low granularity of the stationary phase carrier (sub-2 μm column) in analyzing selected antibiotics. Penicillin G and cefoxitin were selected for testing as representatives of the β-lactam group, and tetracycline as a representative of the tetracycline antibiotic group. A chromatographic column SB-C18 with parameters 4.6 x 50 mm and a filling grain size of 1.8 μm (Phenomenex) was chosen for testing. The column substantially reduced the analysis time (β-lactam 45–74%; tetracycline 50%,) while maintaining the qualitative parameters of the data obtained. A sub-2 μm column was possible without a substantial change in the chromatographic parameters for the specific methods tested.

Keywords: liquid chromatography, penicillin, cefoxitin, tetracycline, sub-2 μm columns

pages: 72-78, online: 2024



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