DOI - Vydavatelství Mendelovy univerzity v Brně

Identifikátory DOI

DOI: 10.11118/978-80-7701-024-5-0053

Spectroscopic Properties of Humic Substances in Permanent Grassland Soil

Jana Plisková1,2, Lubica Pospíšilová1, Pavel Nerušil2, Tomáš Šimon2, Ladislav Menšík2
1 Mendel University in Brno, Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Faculty of AgriSciences, Zemědělská 1, 613 00 Brno, Czech Republic
2 Crop Research Institute, Division of Crop Management Systems, Drnovská 507/73, 161 06 Praha 6 – Ruzyně, Czech Republic

UV-VIS and infrared spectroscopy (FTIR) were used for humic substances quality evaluation. Both methods are widely applied because of their availability, sensitivity, rapidity, and affordability. The calculated spectral indexes assess the stability, hydrophobicity, and content of different functional groups in humic substances’ molecules. Humic substances and humic acids were isolated from Gleyic Fluvisol (locality Jaroměřice, Czech Republic). The soil is under permanent grassland, with intensive management (4 cuts/year) and fertilisation with high-quality farmyard manure (FYM – the cattle loading 2 DJ. ha-1). Soil samples were taken from a depth of 0–0.15 m from control and FYM variants during 2023–2024. Results were statistically evaluated (EDA, ANOVA /Tuckey’s test/). UV-VIS spectra indicated a higher total humic substances content after amending soil with FYM. FTIR spectra showed more aliphatic and hydrophilic components after FYM amendments. Linear correlation was found between humic acid content and organic carbon content.

Klíčová slova: humic substances, grassland, farmyard manure, UV-VIS and FTIR spectroscopy

stránky: 53-58, online: 2025



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