DOI: 10.11118/978-80-7701-093-1-0084

EFFECTS OF LACTIPLANTIBACILLUS PLANTARUM AND PEDIOCOCCUS PENTOSACEUS ON THE FERMENTATION CHARACTERISTICS AND AEROBIC STABILITY OF GRASS/LEGUME SILAGE

Jatkauskas, J.¹, Vrotniakiene, V.¹, Roth, A.²

¹ Lithuanian University of Health Sciences, R. Žebenkos 12, Baisogala, 82317, Lithuania

² Josera GmbH & Co. KG Industriegebiet Süd, 63924 Kleinheubach, Germany, Eingetragen: HRA 2827 Aschaffenburg

This study evaluated the effect of an inoculant (containing Lactiplantibacillus plantarum and Pediococcus pentosaceus in combination with xylanase enzyme) on the chemical composition, fermentation characteristics, and aerobic stability of grass/legume silage. The experiment was conducted using an in vitro mini-silo system in accordance with DLG guidelines, with silages analyzed after 90 days of fermentation. Compared with the untreated control, inoculated silage showed significantly higher contents of dry matter (DM), DM corrected for volatiles (DMc), crude protein (CP), and water-soluble carbohydrates (WSC), as well as reduced overall DM losses during storage. The inoculant significantly improved fermentation quality, as indicated by lower pH values, higher total acid concentrations, and increased levels of individual organic acids, particularly lactic acid. In addition, concentrations of undesirable fermentation products, including ammonia-N, alcohols, and butyric acid, were significantly reduced. Microbiological analysis showed higher lactic acid bacteria counts and reduced populations of yeasts, moulds, and clostridia. During the aerobic stability test, inoculated silage maintained lower pH, lower weight losses, and reduced spoilage microorganisms, resulting in significantly longer aerobic stability. Overall, inoculant application improved fermentation efficiency, preserved nutrients, suppressed undesirable microorganisms, and enhanced the aerobic stability of grass/legume silage.

Klíčová slova: aerobic stability, grass/legume silage, bacterial strains, fermentation, dry matter losses

stránky: 84-85, Publikováno: 2026, online: 2026