DOI: 10.11118/978-80-7509-996-9-0046

ZMĚNY RAMANOVA SPEKTRA PROTEINU SÓJI LUŠTINATÉ VLIVEM RŮZNÝCH NANOČÁSTIC / CHANGES IN THE RAMAN SPECTRUM OF SOY PROTEIN ISOLATE DUE TO VARIOUS NANOPARTICLES

Jana Čaloudová¹, Matej Pospiech¹, Kateřina Křištofová¹, Bohuslava Tremlová¹

¹ Ústav hygieny a technologie potravin rostlinného původu, Fakulta veterinární hygieny a ekologie, Veterinární univerzita Brno, Palackého třída 1948/1, 612 42 Brno

With the increasing trend of replacing animal proteins with plant proteins, the development of methods suitable for reducing the potential allergenicity of soy proteins is becoming increasingly important. One possibility is to reduce the allergenicity of soy protein by interacting with nanoparticles. Raman spectroscopy has demonstrated changes in the intensity of radiation, suggesting the effectiveness of reducing the allergenicity of soy protein. The addition of nanoparticles, especially ferromagnetic (FE) nanoparticles, led to a change in Raman spectra, indicating the formation of new bonds, even at low concentrations (0.15 mg∙g-1). It was further confirmed that the incubation time of the sample has an effect on the Raman spectra. These results may be used in the future for the development of foods containing soy protein with reduced allergenicity. For further research in this area, it would be useful to extend the spectrum of nanoparticles used for the plant proteins studied.

Keywords: calcium phosphate, iron oxide, silicon oxide, nanoparticles, soya protein, Raman spectroscopy, food allergy

pages: 46-53, online: 2024