DOI: 10.11118/978-80-7509-996-9-0216

ZMĚNA RAMANOVA SPEKTRA PROTEINŮ SÓJI LUŠTINATÉ PO INKUBACI S BIOAKTIVNÍMI LÁTKAMI / CHANGE OF RAMAN SPECTRUM OF SOYBEAN PROTEINS AFTER INCUBATION WITH BIOACTIVE SUBSTANCES

Kateřina Křištofová¹, Matej Pospiech¹, Jana Čaloudová¹, Bohuslava Tremlová¹

¹ Ústav hygieny a technologie potravin rostlinného původu, Fakulta veterinární hygieny a ekologie, Veterinární univerzita Brno, Palackého tř. 1946/1, 612 42 Brno

The allergenic proteins in soybeans represent a significant barrier to their full use in the human diet. Allergic reactions can manifest themselves in a variety of symptoms such as skin rashes, respiratory and digestive problems. Soybeans are included in the list of allergens issued by the European Union. The aim of this work is to point out the possibilities of reducing the immunoreactivity of proteins of plant origin, specifically soybean (Glycine max) proteins, by the addition of bioactive substances-gallic acid, quercetin, β-carotene. The samples included different concentrations of bioactive substances - 0;0.15;0.5;1.5 and 15 mg/g. The measurements were performed on a Raman spectrometer. The highest interactions were observed for concentrations of 0.15 and 1.5 mg/g, higher concentrations indicated masking by the substance itself. The addition of gallic acid and quercetin at concentrations of 0.15 and 1.5 mg/g seems to be the most suitable for soybean proteins binding. The mechanism of formation of polyphenol-protein complexes is the subject of further investigations.

Keywords: Raman spectroscopy, soybean, immunoreactivity, bioactive substances

pages: 216-222, online: 2024