DOI - Mendel University Press

DOI identifiers

DOI: 10.11118/978-80-7701-048-1-0161

STANOVENÍ CHOLESTEROLU VE ŽLOUTKU POMOCÍ FT-NIR SPEKTROMETRIE / DETERMINATION OF CHOLESTEROL IN EGG YOLK USING FT-NIR SPECTROSCOPY

Michaela Králová1, Eliška Dračková2, Lenka Kaniová1, Vojtěch Anderle2, Martina Lichovníková2
1 Ústav hygieny a technologie potravin živočišného původu a gastronomie, Fakulta veterinární hygieny a ekologie, Veterinární univerzita Brno, Palackého tř. 1946/1, 612 42 Brno
2 Ústav chovu a šlechtění zvířat, Agronomická fakulta, MENDELU, Zemědělská 1665/1, 613 00 Brno


The aim of this study was to create calibration models for the determination of cholesterol content in egg yolk using the FT-NIRs method. Spectra of samples were obtained by measuring in reflectance mode on an integrating sphere using a transflectance cell in the spectral range of 10,000–4,000 cm-1. Values for creating calibration models were obtained using the UPLC reference method. A reliable calibration model was created using the PLS method. A close relationship was found between the reference and predicted NIR values and no statistically significant difference was found using the paired t-test (p ˃ 0.05). The coefficients of determination R2 were found to be 0.57 for calibration and 0.49 for validation. Therefore, this calibration model is more suitable for indicative determination.

Keywords: near-infrared spectrometry with Fourier transformation, PLS method, egg

pages: 161-166, online: 2025



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